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In MapPropretiesTree, look for the "players" section, and for each player, set "CanBeHumanPlayer" to true (if you want a human player to be able to choose that player number at the start) or false (if not). Also, set "CanBeComputerPlayer" to true (if you want a computer player to be able to play as that player number at the start) or false (if not).