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Waterfall_13, could you share a screenshot of the alpha channel? Maybe the issue is simply with it.
I suspect that the alpha channel might not have been drawn correctly. However, I could be wrong. How did you create the alpha channel? Did you select the black areas in the image and then fill them in on the alpha channel?
If so, try this instead: simply copy the original image and paste it onto the alpha channel. It will become black and white on the alpha channel. Then, adjust the brightness to your liking.
Added 10 minutes later
There's also the possibility that you need to edit the export settings of the image, or the xdb file that controls the texture or material. I don't remember exactly, but there was a function there that controlled how the alpha channel is handled. There are different values, including normal transparency processing or just a hard "visible/not visible" setting. You need to look and compare how these xdb files are configured for different materials and textures.